以二水合乙酸锌、2-甲基咪唑、匡威1970s水蜜桃色官方-免费-完整版片（g-CNQDs，7 mg）和匡威1970s水蜜桃色官方-免费-完整版片（AchE）为起始原料，采用一锅法制备了匡威1970s水蜜桃色官方-免费-完整版片（ZIFs）包载g-CNQDs和AchE的酸度敏感型复合物ZIFs@g-CNQDs@AchE，并和患阿尔茨海默病（AD）小鼠的脑组织样品在pH 7.4磷酸盐缓冲溶液中室温反应2.5 h，在380 nm激发波长下测量体系在475 nm处的荧光强度，用于测定匡威1970s水蜜桃色官方-免费-完整版片（Ach）的含量。结果显示，合成的复合物呈十二面体结构，平均粒径约300 nm，加入Ach后，Ach渗透进入复合物，被AchE催化生成乙酸，体系酸度增加，复合物结构瓦解，g-CNQDs释放，475 nm处出现明显的荧光发射峰。Ach的浓度在5.5~82.5 mmol·L^-1内和对应的体系荧光强度呈线性关系，检出限（3S/N）为1.83 mmol·L^-1；常见的干扰物质（葡萄糖、多巴胺）以及干扰离子（Fe²⁺、Fe³⁺、Cu²⁺、Mg²⁺、Zn²⁺）对Ach的检测无干扰，复合物的特异性较高；按照标准加入法对阳性实际样品进行回收试验，成功检出了Ach，回收率为93.2%~112%，测定值的相对标准偏差（n=5）为0.82%~3.4%。构建的酸度敏感型复合物可作为一种潜在的载体，用于AD诊疗。

Using zinc acetate dihydrate, 2-methylimidazole, graphitic carbon nitride quantum dots (g-CNQDs, 7 mg) and 匡威1970s水蜜桃色官方-免费-完整版片 (AchE) as starting materials, the acidity sensitive complex of zeolitic imidazolate frameworks (ZIFs) containing g-CNQDs and AchE (denoted as ZIFs@g-CNQDs@AchE) was prepared by one pot method. In order to determine Ach, the complex and brain tissues samples of mouse with AD were reacted in the pH 7.4 phosphate buffer solution at room temperature for 2.5 h, and the fluorescence intensity of the system was measured at 475 nm with excitation wavelength of 380 nm. It was shown that the synthesized complex had a dodecahedron structure with an average particle size about 300 nm. After adding Ach, Ach penetrated into the complex and was catalyzed by AchE to generate acetic acid. The acidity of the system increased, the complex structure was collapsed, g-CNQDs were released, and an obvious fluorescence emission peak appeared at 475 nm. The linear relationship between values of the concentration of Ach and the corresponding fluorescence intensity of the system was kept in the range of 5.5-82.5 mmol·L^-1, with detection limit (3S/N) of 1.83 mmol·L^-1. Common interfering substances (glucose and dopamine) and interfering ions (Fe²⁺, Fe³⁺, Cu²⁺, Mg²⁺ and Zn²⁺) did not interfere with the detection of Ach, indicating that the specificity of the complex was high. Test for recovery was made according to standard addition method on the positive actual samples, and Ach was successfully detected, giving recoveries in the range of 93.2%-112% and RSD (n=5) of the determined values in the range of 0.82%-3.4%. The constructed acidity sensitive complex can serve as a potential carrier for the diagnosis and treatment of AD.